Molecular Cloning of Streptomyces Genes Encoding Vanillate Demethylase

M. Nishimura, D. Ishiyama, J. Davies

Bioscience, biotechnology, and biochemistry, 2006

The vanillate demethylase genes from Streptomyces sp. NL15-2K were cloned and sequenced. The vanA and vanB gene homologs, which encode the terminal oxygenase subunit (VanA) and the ferredoxin-type reductase subunit (VanB) of the enzyme respectively, were found in the sequenced 7.5-kb DNA region. Expression of the vanAB genes in Streptomyces lividans 1326 resulted in in vivo demethylation of veratric acid to vanillic acid.

Cited by 3 publications.

Field of study: Biology

10.1271/bbb.60180