R. Edwards, K. Mansouri, V. Stalls, K. Manne, Brian E Watts, R. Parks, K. Janowska, S. Gobeil, Megan F. Kopp, Dapeng Li, X. Lu, Z. Mu, Margaret Deyton, T. Oguin, Jordan Sprenz, W. Williams, K. Saunders, D. Montefiori, G. Sempowski, R. Henderson, Munir S. Alam, B. Haynes, P. Acharya
bioRxiv : the preprint server for biology, 2020
The SARS-CoV-2 spike (S) protein, a primary target for COVID-19 vaccine development, presents its Receptor Binding Domain in two conformations: receptor-accessible “up” or receptor-inaccessible “down” conformations. Here, we report that the commonly used stabilized S ectodomain construct “2P” is sensitive to cold temperature, and that this cold sensitivity is resolved in a “down” state stabilized spike. Our results will impact structural, functional and vaccine studies that use the SARS-CoV-2 S ectodomain.
Cited by 14 publications.
Field of study: Medicine