Jon Arizti-Sanz, C. A. Freije, A. Stanton, Chloe K. Boehm, Brittany A. Petros, Sameed M Siddiqui, B. Shaw, Gordon C. Adams, Tinna-Solveig F. Kosoko-Thoroddsen, Molly E Kemball, R. Gross, L. Wronka, Katie Caviness, L. Hensley, N. Bergman, B. MacInnis, J. Lemieux, Pardis C Sabeti, Cameron Myhrvold
bioRxiv : the preprint server for biology, 2020
The COVID-19 pandemic has highlighted that new diagnostic technologies are essential for controlling disease transmission. Here, we develop SHINE (SHERLOCK and HUDSON Integration to Navigate Epidemics), a sensitive and specific integrated diagnostic tool that can detect SARS-CoV-2 RNA from unextracted samples. We combine the steps of SHERLOCK into a single-step reaction and optimize HUDSON to accelerate viral inactivation in nasopharyngeal swabs and saliva. SHINE’s results can be visualized with an in-tube fluorescent readout — reducing contamination risk as amplification reaction tubes remain sealed — and interpreted by a companion smartphone application. We validate SHINE on 50 nasopharyngeal patient samples, demonstrating 90% sensitivity and 100% specificity compared to RT-PCR with a sample-to-answer time of 50 minutes. SHINE has the potential to be used outside of hospitals and clinical laboratories, greatly enhancing diagnostic capabilities.
Cited by 17 publications.
Field of study: Medicine