A. N. Mohon, J. Hundt, G. van Marle, K. Pabbaraju, B. Berenger, T. Griener, L. Lisboa, D. Church, M. Czub, A. Greninger, K. Jerome, C. Doolan, D. Pillai
medRxiv, 2020
We have developed a reverse-transcriptase loop mediated amplification (RT-LAMP) method targeting genes encoding the Spike (S) protein and RNA-dependent RNA polymerase (RdRP) of SARS-CoV-2. The LAMP assay achieves the same limit of detection as commonly used RT-PCR protocols based on artificial targets, recombinant Sindbis virus, and clinical samples. Clinical validation of single target LAMP (N=108) showed a positive percent agreement (PPA) of 33/34 (97.1%) and negative percent agreement (NPA) of 73/74 (98.6%) compared to reference RT-PCR. Dual target RT-LAMP achieved a PPA of 11/11 (100%) and NPA 13/13 (100%) when including discrepant samples. The assay can be performed without a formal extraction procedure, with lyophilized reagents that do need cold chain, and is amenable to point-of-care application with visual detection.
Cited by 5 publications.
Field of study: Chemistry