Qunxiong Pan, Jiangman Zhao, Mingzhu Li, Xiaoyu Liu, Ya-ping Xu, Wushuang Li, Shouxin Wu, Zi-jian Su
Carcinogenesis, 2019
An accurate biomarker or method for diagnosis of thyroid nodule with indeterminate fineneedle aspiration result is essential for clinical treatment. miRNAs of exosomes are advantageous in the diagnosis of tumors because they are highly stable, and be protected by a bilayer membrane structure. Exosomes were isolated from 13 papillary thyroid carcinoma (PTC) and 7 nodular goiter (NG) patients' plasma. Small RNA sequencing was performed on exosomes' RNA in next-generation sequencing (NGS) platform. Then we performed comprehensive analysis on miRNA expression profile in exosome of two groups. 129 differential expressed miRNAs were identified in plasma exosomes between PTC and NG patients. 49 miRNAs were up-regulated, and 80 miRNAs were down-regulated in PTC patients. Receiver Operating Characteristic (ROC) curves of 129 miRNAs were plotted. Area Under Curve (AUC) of 129 miRNAs was 0.571-0.951, with distribution peak of 0.82-0.86. AUC of 11 miRNAs was above 0.9, miR-5189-3p had the most optimal performance for diagnosis between PTC and NG, with 0.951 of AUC. Target genes of 129 miRNAs were enriched into 7 cancer-related signaling pathways, including MAPK, TNF, NF-kappa B signaling pathway et al. This study profiled the miRNA signature of exosomes from PTC patients and NG patients. We proposed a group of miRNAs in plasma exosomes as candidate biomarkers for thyroid nodule diagnosis.
Cited by 8 publications.
Field of study: Medicine