F. Sun, A. Ganguli, J. Nguyen, Ryan P. Brisbin, Krithika Shanmugam, D. L. Hirschberg, Matthew B Wheeler, R. Bashir, David M. Nash, B. Cunningham
Lab on a chip, 2020
Rapid, sensitive and specific detection and reporting of infectious pathogens is important for patient management and epidemic surveillance. We demonstrated a point-of-care system integrated with a smartphone for detecting live virus from nasal swab media, using a panel of equine respiratory infectious diseases as a model system for corresponding human diseases such as COVID-19. Specific nucleic acid sequences of five pathogens were amplified by loop-mediated isothermal amplification on a microfluidic chip and detected at the end of reactions by the smartphone. Pathogen-spiked horse nasal swab samples were correctly diagnosed using our system, with a limit of detection comparable to that of the traditional lab-based test, polymerase chain reaction, with results achieved in ∼30 minutes.
Cited by 30 publications.
Field of study: Medicine